High Resolution Mass determination, separation, qualitative and quantitative analysis by liquid chromatography Mass spectroscopy LC(UV)MS/MS using Agilent 6520 QTOF analyzer. High-resolution LCMS/MS Quadruple Time of Flight is an identification tool which allows distinguishing peaks separated by only small m/z values and provides excellent mass accuracy < 2ppm for MS and < 4 ppm for MS/MS also allows selective detection of desired compounds in the presence of interferences. Liquid Chromatography Mass Spectrometry enables chromatographic separation, identification and quantification of compounds in a wide range of masses (50-20000). Ionization of the samples occurs in Dual Electro Spray Ionization source which is suitable for compounds that can have a charge induced and for multiply charged compounds such as: proteins, peptides, oligonucleotides. The samples for this analyzer have to be soluble in polar well vaporizable solvents.